翻訳と辞書 |
Blotto (biology) : ウィキペディア英語版 | Blotto (biology) In biology, BLOTTO is a blocking reagent made from nonfat dry milk, phosphate buffered saline, and sodium azide. Its name is an almost-acronym of Bovine Lacto Transfer Technique Optimizer. It constitutes an inexpensive source of nonspecific protein (milk casein) which blocks protein binding sites in a variety of experimental paradigms, notably Southern blots, Western blots, and ELISA. Its use was first reported in 1984 by Johnson and Elder's lab at Scripps. Prior to 1984, partially purified proteins such as bovine serum albumin, ovalbumin, or gelatin from various species had been used as blocking reagents but had the disadvantage of being expensive. ==References==
抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)』 ■ウィキペディアで「Blotto (biology)」の詳細全文を読む
スポンサード リンク
翻訳と辞書 : 翻訳のためのインターネットリソース |
Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.
|
|